Fig. 3

Penetrating vasculature in the Hspg2^∆3∆91 mice. A, C) Brain sections from mice euthanized 30 min after OVA-TxRed tracer (white) infusion into the cisterna magna were co-stained with ERTR7 (red), Col IV (blue) and aSMA (green). B, D) Enlarged insets from A and C respectively, showing the distribution of tracer (white) and its co-localization with ERTR7 and Col IV⁺aSMA⁺ vessels (arteries, white arrowhead). E, F) Numbers of ERTR7 and tracer-positive vessels and their distribution based on location in Hspg2^+/+ (n=6) and Hspg2^∆3∆91 (n=6) mice. G-I) Quantification of penetration lengths of ERTR7, tracer, and their relative distance along with their distribution based on their location in the Hspg2^+/+ (n=6) and Hspg2^∆3∆91 (n=6) mice. Scale bar; 1 mm (A, C), 50 µm (B, D). The statistical test used was 2-way ANOVA followed by Sidak’s post-hoc test (E-I). *p < 0.05, ***p < 0.001, ****p < 0.0001. ns, non-significant. Mean ± SEM