Fig. 2

Deletion of glycosaminoglycan chain attachment sites from the perlecan core protein delays cerebrospinal fluid influx into the brain. A) Schematic timeline for the experiment and sample collection. B) Coronal sections of mice brains for the time points indicated, showing the presence of OVA-TxRed tracer (magenta) in the perivascular spaces of the Hspg2^+/+ and Hspg2^∆3∆91 mice. White arrowheads indicating the locations with reduced perivascular influx in Hspg2^∆3∆91 as compared to Hspg2^+/+. Enlarged images of brain sections highlighting reduced tracer (magenta) in dorsal (D) and ventral (D’) locations in Hspg2^∆3∆91. E) Quantification of the tracer area showing a statistically significant difference between Hspg2^+/+ and Hspg2^∆3∆91 at 30 min (n = 6) but not at 60 min (n = 6) or 90 min (n = 5–6 mice per genotype). Intracranial pressure (ICP) measurements did not show any difference between the Hspg2^+/+ (n = 10) and Hspg2^∆3∆91 (n = 11) mice. Scale bar, 1 mm (B). The statistical tests used were 2-way ANOVA followed by Sidak’s post-hoc test (E) and the Mann-Whitney unpaired two-tailed t-test (F).  *p < 0.05, **p < 0.01. ns, non-significant. Mean ± SEM