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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: A face-to-face comparison of claudin-5 transduced human brain endothelial (hCMEC/D3) cells with porcine brain endothelial cells as blood–brain barrier models for drug transport studies

Fig. 5

Concentration equilibrium transport assay (CETA) of Pgp substrate N-desmethyl-loperamide (dLop) in hCMEC/D3-WT, hCMEC/D3-Cldn5-YFP and pBCECs. Data are shown as percentage of the initial drug concentration (= 100%) in the apical and basolateral chamber of the Thincert system over time. a An increase of the drug concentration in the apical chamber and a simultaneous reduction in the basolateral chamber indicates drug transport by Pgp across the primary pBCEC monolayer. Almost no drug transport was measurable when the experiment was repeated in the presence of the Pgp inhibitor verapamil (50 µM) as a control. b, c A Pgp-mediated ([3H]dLop) transport across the hCMEC/D3 monolayer could not be observed

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Fluids and Barriers of the CNS

ISSN: 2045-8118