Fig. 2

Tight junction proteins. a Quantitative mRNA expression levels of F11R. There were no significant differences in expression levels. b Quantitative mRNA expression levels of TJP1. There were no specific differences in expression levels. c Quantitative mRNA expression levels of OCLN. OCLN was significantly downregulated in MC and NCC compared to capillaries. d Quantitative mRNA expression levels of CLDN5. There were no significant differences in expression levels. e Quantitative expression levels of CLDN1. CLDN1 expression levels were low in all culture configurations. n = 3, N = 3. F: Trans-endothelial electrical resistance (TEER) in the different models, n = 4 − 6, N = 4 − 17. Please note that the y-axis are different in all graphs. F11R: JAM-1, TJP1: Zo-1, OCLN: Occludin, CLDN5: Claudin-5, CLDN1: Claudin-1, MC: Mono culture, NCC: Non-contact co-culture, CCC: Contact co-culture. *: P ≤ 0.05, **: P ≤ 0.005. Error bars are mean + SEM